WP8010: Determination of Multiple Quality Attributes of Antibody-Oligo Conjugate with SEC-MALS and AEX-MALS

Liquid chromatography combined with multi-angle light scattering (LC-MALS) is a powerful tool for analyzing antibody-oligonucleotide conjugates (AOCs), providing a robust, straightforward approach to obtaining multiple quality attributes. In this application note, we demonstrate the successful application of LC-MALS with both size exclusion chromatography (SEC) and anion ex-change chromatography (AEX) to quantify the oligo-antibody ratio (OAR) and overall payload concentration of two AOC samples. Through a single LC-MALS experiment, we identify the complex molecular species pre-sent in the samples, including aggregates, fragments, and different OAR populations.