Multi-Angle Light Scattering (MALS) Detectors
DAWN™
The world's most advanced MALS instrument for characterization of macromolecules and nanoparticles in solution to determine absolute molar mass and size.
In line with HPLC for SEC-MALS, downstream of Eclipse field-flow fractionation, stand alone or with a Calypso, the DAWN offers the highest sensitivity and widest range of measurements for peptides, proteins, conjugates, polymers, and nanoparticles. Optional configurations include temperature control, embedded dynamic light scattering and automated flow-cell cleaning.
miniDAWN™
The best in basic multi-angle light scattering for absolute macromolecular analysis
The miniDAWN determines absolute molar mass and size to characterize proteins, peptides, small polymers and nanoparticles in solution. Coupled to SEC/GPC, it eliminates the uncertainty and hassle of column calibration, for true confidence in macromolecular characterization.
microDAWN™
The first SEC-MALS detector for UHPLC. Uncompromised sensitivity.
The microDAWN can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of proteins, peptides, polymers or nanoparticles below 50 nm in radius directly, without resorting to column calibration or reference standards.
The microDAWN brings all the advantages of SEC-MALS to the world of UHPLC, including optional embedded dynamic light scattering. Use in conjunction with the Optilab differential refractometer for UHPLC.
ultraDAWN™
A breakthrough in process analytical technology for production of nanoparticles, biopharmaceuticals and polymers.
For the first time, critical quality attributes, or CQAs – including weight-average molecular weight Mw and rms radius Rg – can be monitored directly, in-line or on-line, for rapid feedback on product and process quality.
Feature Comparison
| Capabilities | DAWN™ | miniDAWN™ | microDAWN™ | ultraDAWN™ |
| Environment | Analytical HPLC/FPLC | Analytical HPLC/FPLC | Analytical UHPLC | Process |
| Integrated DLS module | Optional WyattQELS™ | Optional WyattQELS | Optional WyattQELS | No |
| DLS fiber connection | Optional, to NanoStar™ or ZetaStar™ | Optional, to NanoStar or ZetaStar | Optional, to NanoStar or ZetaStar | Not available |
| Measurements | ||||
| Molar Mass Range | 200 Da to 1 GDa | 200 Da to 10 MDa | 200 Da to 10 MDa | 1 kDa to 1 GDa |
| Molecular Size Range (Rg) |
10 to 500 nm, up to 1000 nm with shape-specific models | 10 to 50 nm, up to 150 nm with shape-specific models |
10 to 50 nm, up to 150 nm with shape-specific models |
10 to 250 nm |
| Molecular Size Range (Rh, with DLS)1 |
Flow: 0.5 to 300 nm Batch: 0.5 nm to 1 µm |
Flow: 0.5 to 50 nm Batch: 0.5 nm to 1 µm |
Flow: 0.5 to 30 nm 0.5 nm to 1 µm |
N/A |
| MALS Sensitivity | 10 ng of 100 kDa polystyrene in THF2; 200 ng of BSA in PBS3 |
25 ng of 100 kDa polystyrene in THF2; 500 ng of BSA in PBS3 |
3.5 ng of 100 kDa polystyrene in THF4; 70 ng of BSA in PBS4 |
Process Dependent |
| Optics | ||||
| Static Scattering Detectors | 18 angles | 3 angles | 3 angles | 18 angles |
| Laser Power Control | Programmable 10% to 100% | Programmable 10% to 100% | Programmable 10% to 100% | Programmable 10% to 100% |
| DLS Scattering Angle (optional WyattQELS) |
any one of 4 dedicated locations | 135° | 130° | N/A |
| Temperature Options | Ambient Ultra-High to +210 °C Heated/Cooled -15 °C to +150 °C |
Ambient only | Ambient only | Ambient only |
| Host Software | ASTRA™ | ASTRA | ASTRA | OBSERVER™ |
1Size range will depend on flow rate, application, and instrument configuration.
2Under HPLC-SEC conditions with 7.8x300 mm columns and no post-column filter.
3Under HPLC-SEC conditions with 7.8x300 mm Wyatt SEC protein columns and no post-column filter.
4Under UHPLC-SEC conditions with 4.6x150 mm columns assuming a 15 cm UHPLC-SEC column and no post-column filter.